Oxidative activation of 2-aminofluorene by human red blood cell cytosol.
Biochem Pharmacol
; 44(12): 2422-4, 1992 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-1472110
ABSTRACT
Purified red blood cell cytosol is able to activate 2-aminofluorene (2AF) to N-hydroxy-2-aminofluorene. Apparent kinetic parameters are determined with and without methylene blue. The latter, which maintains haemoglobin in the reduced form and stimulates NADPH production, increases the affinity of the enzyme for the 2AF. This activity is inhibited by carbon monoxide while cyanide is without effect. The involvement of a peroxidative reaction or a one-electron oxidative mechanism involving free radicals may be excluded.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Erythrocytes
/
Fluorenes
Limits:
Humans
/
Male
Language:
En
Journal:
Biochem Pharmacol
Year:
1992
Document type:
Article
Affiliation country:
Belgium