Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formation.

Gunsior, Michele; Ravel, Jacques; Challis, Gregory L; Townsend, Craig A

Gunsior, Michele; Ravel, Jacques; Challis, Gregory L; Townsend, Craig A.

Affiliation

Gunsior M; Department of Chemistry, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA.

Biochemistry ; 43(3): 663-74, 2004 Jan 27.

Article in En | MEDLINE | ID: mdl-14730970

Subject(s)

4-Hydroxyphenylpyruvate Dioxygenase/chemistry; Alkyl and Aryl Transferases/chemistry; Cyclohexanes/chemistry; Glycine/analogs & derivatives; Homogentisic Acid/chemistry; Mandelic Acids/chemistry; Protein Engineering/methods; 4-Hydroxyphenylpyruvate Dioxygenase/genetics; 4-Hydroxyphenylpyruvate Dioxygenase/isolation & purification; Alkyl and Aryl Transferases/genetics; Catalysis; Glycine/chemistry; Models, Molecular; Mutagenesis, Site-Directed; Phylogeny; Pseudomonas fluorescens/enzymology; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Sequence Homology, Amino Acid; Streptomyces/enzymology

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Collection: 01-internacional Database: MEDLINE Main subject: Protein Engineering / Alkyl and Aryl Transferases / Cyclohexanes / Glycine / Homogentisic Acid / 4-Hydroxyphenylpyruvate Dioxygenase / Mandelic Acids Language: En Journal: Biochemistry Year: 2004 Document type: Article Affiliation country: United States Country of publication: United States

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