[Determination of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis].
Yao Xue Xue Bao
; 38(11): 843-5, 2003 Nov.
Article
in Zh
| MEDLINE
| ID: mdl-14991998
ABSTRACT
AIM:
To establish a method for determinate of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis.METHODS:
The characteristic absorptive wavelength of hippuric acid determined by ultraviolet spectrophotometer is 228 nm. The method employed a melted capillary column, 50 mmol.L-1 phosphoric acid (pH 8.3) buffer solution, inject pressure 4.8 kPa, inject time 3 s, separation voltage 20 kV and detection wavelength 228 nm.RESULTS:
The reactant and resultant was separated completed within 7 min. IC50 of captopril was 0.019 mumol.L-1. Captopril is a competitive inhibitor, which was proved by enzyme reaction dynamics.CONCLUSION:
The method was shown to be accurate, simple and rapid and can be used for determination of the inhibitory activity of captopril.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Angiotensin-Converting Enzyme Inhibitors
/
Captopril
/
Peptidyl-Dipeptidase A
/
Hippurates
Language:
Zh
Journal:
Yao Xue Xue Bao
Year:
2003
Document type:
Article
Affiliation country:
China