Determination of procaine in equine plasma and urine by high-performance liquid chromatography.
J Anal Toxicol
; 16(2): 93-6, 1992.
Article
in En
| MEDLINE
| ID: mdl-1501471
ABSTRACT
The variability in plasma and urine equine procaine measurement between three independent laboratories using current methods led to the development of a sensitive, reliable, and reproducible high-performance liquid chromatographic method. Standardbred mares were administered either a penicillin G procaine preparation intramuscularly or procaine hydrochloride subcutaneously, and blood and urine were collected at defined time intervals. By HPLC the detection limits for procaine in plasma and urine were 1 and 10 ng/mL, respectively. In contrast procaine in plasma could not be detected by GC-NPD, while the urinary detection limit was 50 ng/mL. The concentration of fluoride in the collection tubes and repetitive freeze-thawing modified plasma procaine measurement. Urinary pH was a factor in estimation of urine procaine levels with greater recovery and reproducibility of results at pH 5 as compared to pH 7. This HPLC method provides a simple, sensitive, and reliable quantitation of procaine in equine plasma and urine.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Procaine
/
Chromatography, High Pressure Liquid
/
Horses
Limits:
Animals
Language:
En
Journal:
J Anal Toxicol
Year:
1992
Document type:
Article