Structure of Lipid A from Pseudomonas corrugata by electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
Rapid Commun Mass Spectrom
; 18(8): 853-8, 2004.
Article
in En
| MEDLINE
| ID: mdl-15095352
ABSTRACT
The use of the electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-QTOFMS) technique for the structural determination of Lipid A from Pseudomonas corrugata is described. This technique appears to be more sensitive with respect to other commonly used tandem mass spectrometric approaches, and was very valuable in the structural determination of the highly heterogeneous Lipid A fractions. The Lipid A fraction consists mainly of a pentaacyl component in which 3-hydroxydecanoyl [100(3-OH)] and 3-hydroxydodecanoyl [120(3-OH)] are linked as primary acyl substituents to the classical bisphosphorylated beta-(1' --> 6)-linked D-glucosamine disaccharide. Secondary substitution of N-acyl fatty acids with dodecanoyl residues [120] and/or its 2-OH derivatives was also observed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Spectrometry, Mass, Electrospray Ionization
/
Lipid A
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2004
Document type:
Article
Affiliation country:
Italy