A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR.
Malar J
; 3: 9, 2004 May 07.
Article
in En
| MEDLINE
| ID: mdl-15132750
ABSTRACT
BACKGROUND:
Surveillance for drug-resistant Plasmodium falciparum should be a component of malaria control programmes. Real-time PCR methods for the detection of parasite single-nucleotide polymorphisms (SNPs) and gene amplification could be useful survellance tools.METHODS:
A real-time PCR assay has been developed that identifies single nucleotide polymorphisms (SNPs) at amino acids 86, 184, 1034 and 1042 in the P. falciparum multi-drug resistant (pfmdr 1) gene that may be associated with anti-malarial drug resistance.RESULTS:
This assay has a sensitivity and specificity of 94% and 100% when compared to traditional PCR methods for genotyping. Only 54 of 68 (79%) paired pre- and post-culture DNA samples were concordant at all four loci.CONCLUSION:
Real-time PCR is a sensitive and specific method to detect SNP's in pfmdr 1. Genotypes of parasites after in vitro culture may not reflect that seen in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Polymerase Chain Reaction
/
Malaria, Falciparum
/
Genes, MDR
/
Polymorphism, Single Nucleotide
/
Antimalarials
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Malar J
Journal subject:
MEDICINA TROPICAL
Year:
2004
Document type:
Article
Affiliation country:
United States