Tight regulation from a single tet-off rAAV vector as demonstrated by flow cytometry and quantitative, real-time PCR.

Jiang, L; Rampalli, S; George, D; Press, C; Bremer, E G; O'Gorman, M R G; Bohn, M C

Jiang, L; Rampalli, S; George, D; Press, C; Bremer, E G; O'Gorman, M R G; Bohn, M C.

Affiliation

Jiang L; 1Department of Pediatrics, Children's Memorial Institute for Education & Research, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Gene Ther ; 11(13): 1057-67, 2004 Jul.

Article in En | MEDLINE | ID: mdl-15152187

Subject(s)

Central Nervous System/metabolism; Dependovirus/genetics; Gene Expression Regulation; Genetic Therapy/methods; Genetic Vectors/genetics; Neurodegenerative Diseases/therapy; Animals; Cell Line; Doxycycline/pharmacology; Flow Cytometry; Gene Expression; Gene Expression Regulation/drug effects; Genetic Vectors/administration & dosage; Green Fluorescent Proteins; HeLa Cells; Humans; Luminescent Proteins/genetics; RNA, Messenger/analysis; Rats; Rats, Inbred F344; Recombinant Proteins/administration & dosage; Recombinant Proteins/genetics; Reverse Transcriptase Polymerase Chain Reaction; Tetracycline; Transgenes

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Collection: 01-internacional Database: MEDLINE Main subject: Genetic Therapy / Central Nervous System / Gene Expression Regulation / Dependovirus / Neurodegenerative Diseases / Genetic Vectors Limits: Animals / Humans Language: En Journal: Gene Ther Journal subject: GENETICA MEDICA / TERAPEUTICA Year: 2004 Document type: Article Affiliation country: United States Country of publication: United kingdom

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