TRPV1 acts as proton channel to induce acidification in nociceptive neurons.
J Biol Chem
; 279(33): 34553-61, 2004 Aug 13.
Article
in En
| MEDLINE
| ID: mdl-15173182
ABSTRACT
The low extracellular pH of inflamed or ischemic tissues enhances painful sensations by sensitizing and activating the vanilloid receptor 1 (TRPV1). We report here that activation of TRPV1 results in a marked intracellular acidification in nociceptive dorsal root ganglion neurons and in a heterologous expression system. A characterization of the underlying mechanisms revealed a Ca(2+)-dependent intracellular acidification operating at neutral pH and an additional as yet unrecognized direct proton conductance through the poorly selective TRPV1 pore operating in acidic extracellular media. Large organic cations permeate through the activated TRPV1 pore even in the presence of physiological concentrations of Na(+), Mg(2+), and Ca(2+). The wide pore and the unexpectedly high proton permeability of TRPV1 point to a proton hopping permeation mechanism along the water-filled channel pore. In acidic media, the high relative proton permeability through TRPV1 defines a novel proton entry mechanism in nociceptive neurons.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protons
/
Receptors, Drug
/
Nociceptors
/
Neurons
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
J Biol Chem
Year:
2004
Document type:
Article
Affiliation country:
Germany