Labeling of fusion proteins with synthetic fluorophores in live cells.
Proc Natl Acad Sci U S A
; 101(27): 9955-9, 2004 Jul 06.
Article
in En
| MEDLINE
| ID: mdl-15226507
ABSTRACT
A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Recombinant Fusion Proteins
/
O(6)-Methylguanine-DNA Methyltransferase
/
Fluorescent Dyes
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2004
Document type:
Article
Affiliation country:
Switzerland