Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay.
Microb Ecol
; 47(1): 41-7, 2004 Jan.
Article
in En
| MEDLINE
| ID: mdl-15259268
ABSTRACT
Pseudomonas aeruginosa is a pathogenic bacterium that has been thoroughly investigated since the 19th century and is generally regarded as a freshwater or terrestrial organism. In 1995, it was reported that the OprP porin, an outer membrane protein corresponding to that of this bacterium, was widely distributed as a dissolved component in seawater. This finding led us to investigate the presence of P. aeruginosa in marine environments. Both culture-independent and -dependent methods were applied to seawater samples obtained in Tokyo Bay during four cruises. The DVC-FA (direct viable count-fluorescent antibody) technique showed that cells reactive to an antibody against P. aeruginosa were widely present in the bay, i.e., 10(3) to 10(4) cells/mL in the inner bay, and 10(2) to 10(3) cells/mL at the mouth. Bacterial cells isolated by selective medium were identified by three methods:
the presence of oprI and oprL, two outer membrane lipoprotein genes specific to P. aeruginosa; the API20 NE kit; and 16S rDNA sequence analysis. The results confirmed that the majority of isolates from the bay were P. aeruginosa. Immuno-chemical analyses of the seawater results indicate that P. aeruginosa is commonly present in coastal marine environments and sheds OprP.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas aeruginosa
/
Seawater
Type of study:
Prognostic_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Microb Ecol
Year:
2004
Document type:
Article
Affiliation country:
Japan