Purification and characterization of recombinant human cathepsin E expressed in human kidney cell line 293.

Cappiello, Maria G; Wu, Zhongren; Scott, Boyd B; McGeehan, Gerard M; Harrison, Richard K

Cappiello, Maria G; Wu, Zhongren; Scott, Boyd B; McGeehan, Gerard M; Harrison, Richard K.

Affiliation

Cappiello MG; Discovery Biology, Concurrent Pharmaceuticals, 502 W Office Center Drive, Fort Washington, PA 19034, USA.

Protein Expr Purif ; 37(1): 53-60, 2004 Sep.

Article in En | MEDLINE | ID: mdl-15294281

Subject(s)

Cathepsin E/isolation & purification; Cathepsin E/metabolism; Cathepsins/isolation & purification; Cathepsins/metabolism; Enzyme Precursors/isolation & purification; Enzyme Precursors/metabolism; Kidney/cytology; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Amino Acid Sequence; Cathepsin E/chemistry; Cathepsin E/genetics; Cathepsins/chemistry; Cathepsins/genetics; Cell Line; Cloning, Molecular; Enzyme Activation; Enzyme Precursors/chemistry; Enzyme Precursors/genetics; Humans; Hydrogen-Ion Concentration; Molecular Sequence Data; Molecular Structure; Molecular Weight; Recombinant Proteins/chemistry; Recombinant Proteins/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Cathepsins / Cathepsin E / Enzyme Precursors / Kidney Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2004 Document type: Article Affiliation country: United States Country of publication: United States

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