Insulin-sperm interaction: effects on plasma membrane and binding to acrosome.
Arch Androl
; 28(3): 201-11, 1992.
Article
in En
| MEDLINE
| ID: mdl-1530369
In in vitro studies, viable, intact human spermatozoa took up free radioinsulin with an apparently non-receptor-mediated mechanism. However, when a colloidal gold-insulin complex was substituted for the radiotracer, no surface binding was visualized at the ultrastructural level. Upon sperm incubation in the presence of free insulin, a dose-dependent release of phospholipid phosphorus occurred, with a concomitant derangement of head cell membrane. After head membrane removal, spermatozoa-bound radioinsulin in a time- and concentration-dependent manner, the binding was displaceable by unlabeled insulin, and an exclusive localization of the colloidal gold-insulin complex was visualized at the acrosome level. On the basis of this evidence, both the plasma membrane and the acrosome seem to represent cytological targets for insulin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatozoa
/
Acrosome
/
Receptor, Insulin
/
Insulin
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Arch Androl
Year:
1992
Document type:
Article
Affiliation country:
Italy
Country of publication:
United kingdom