Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation.
J Infect Dis
; 190(6): 1127-31, 2004 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-15319863
ABSTRACT
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients--1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Viral
/
Membrane Glycoproteins
/
Viral Envelope Proteins
/
Nucleocapsid Proteins
/
Severe Acute Respiratory Syndrome
/
Severe acute respiratory syndrome-related coronavirus
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Infect Dis
Year:
2004
Document type:
Article
Affiliation country:
United States