Arginine methylation of NIP45 modulates cytokine gene expression in effector T lymphocytes.
Mol Cell
; 15(4): 559-71, 2004 Aug 27.
Article
in En
| MEDLINE
| ID: mdl-15327772
ABSTRACT
Posttranslational modification of proteins within T cell receptor signaling cascades allows T lymphocytes to rapidly initiate an appropriate immune response. Here we report a role for arginine methylation in regulating cytokine gene transcription in the T helper lymphocyte. Inhibition of arginine methylation impaired the expression of several cytokine genes, including the signature type 1 and type 2 helper cytokines, interferon gamma, and interleukin-4. T cell receptor signaling increased expression of the protein arginine methyltransferase PRMT1, which in turn methylated the nuclear factor of activated T cells (NFAT) cofactor protein, NIP45. Arginine methylation of the amino terminus of NIP45 modulated its interaction with NFAT and resulted in augmented cytokine production, while T cells from mice lacking NIP45 had impaired expression of IFNgamma and IL-4. Covalent modification of NIP45 by arginine methylation is an important mechanism of regulating the expression of NFAT-dependent cytokine genes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginine
/
Protein-Arginine N-Methyltransferases
/
Nuclear Proteins
/
Carrier Proteins
/
Gene Expression Regulation
/
Cytokines
/
T-Lymphocytes, Helper-Inducer
/
Intracellular Signaling Peptides and Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
Mol Cell
Journal subject:
BIOLOGIA MOLECULAR
Year:
2004
Document type:
Article
Affiliation country:
United States