Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays.

Achenbach, Jenna E; Topliff, Christina L; Vassilev, Ventzislav B; Donis, Ruben O; Eskridge, Kent M; Kelling, Clayton L

Achenbach, Jenna E; Topliff, Christina L; Vassilev, Ventzislav B; Donis, Ruben O; Eskridge, Kent M; Kelling, Clayton L.

Affiliation

Achenbach JE; Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583-0905, USA.

J Virol Methods ; 121(1): 1-6, 2004 Oct.

Article in En | MEDLINE | ID: mdl-15350726

Subject(s)

Respiratory Syncytial Virus, Bovine/genetics; Respiratory Syncytial Virus, Bovine/isolation & purification; Reverse Transcriptase Polymerase Chain Reaction/methods; Virology/methods; Animals; Cattle; Cell Line; Fluorescence; Fluorescent Dyes/metabolism; RNA, Messenger/analysis; RNA, Viral/analysis; Reference Standards; Reverse Transcriptase Polymerase Chain Reaction/standards; Sensitivity and Specificity; Time Factors

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Collection: 01-internacional Database: MEDLINE Main subject: Virology / Respiratory Syncytial Virus, Bovine / Reverse Transcriptase Polymerase Chain Reaction Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals Language: En Journal: J Virol Methods Year: 2004 Document type: Article Affiliation country: United States Country of publication: Netherlands

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