Molecular typing of Staphylococcus aureus isolates from an intensive care unit.
New Microbiol
; 27(3): 293-9, 2004 Jul.
Article
in En
| MEDLINE
| ID: mdl-15460533
ABSTRACT
Seventeen S. aureus clinical isolates, collected from an Intensive Care Unit (ICU) during a seven-month period were analyzed to investigate their antimicrobial susceptibility and clonal diversity. Eleven isolates (65%) were found to be resistant to methicillin (MRSA). Pulsed-field gel electrophoresis (PFGE) profiles of genomic DNAs, and analysis of the polymorphisms of the variable regions of the protein A (spa) and coagulase (coa) genes revealed a lower clonal heterogeneity among MRSA than among methicillin-susceptible isolates (MSSA). Two of the MRSA clones were repeatedly isolated in different patients, within a variable period of time, suggesting the presence in the ward of a resident, endemic and multi-drug resistant MRSA population. Our results also emphasize the lower discriminatory power of spa and coa typing compared with PFGE typing.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staphylococcal Infections
/
Staphylococcus aureus
/
Bacterial Typing Techniques
/
Intensive Care Units
Country/Region as subject:
Europa
Language:
En
Journal:
New Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2004
Document type:
Article
Affiliation country:
Italy