The role of thioredoxin and disulfide isomerase in the expression of the snake venom thrombin-like enzyme calobin in Escherichia coli BL21 (DE3).

Yuan, Shengling; Duan, Haiqing; Liu, Chunjie; Liu, Xiuli; Liu, Taotao; Tao, Haoxia; Zhang, Zhaoshan

Yuan, Shengling; Duan, Haiqing; Liu, Chunjie; Liu, Xiuli; Liu, Taotao; Tao, Haoxia; Zhang, Zhaoshan.

Affiliation

Yuan S; Beijing Institute of Biotechnology, 20 Dong Dajie Street, Fengtai District, Beijing 100071, China.

Protein Expr Purif ; 38(1): 51-60, 2004 Nov.

Article in En | MEDLINE | ID: mdl-15477082

Subject(s)

Protein Disulfide-Isomerases/metabolism; Serine Endopeptidases/metabolism; Snake Venoms/metabolism; Snakes/metabolism; Thioredoxins/metabolism; Animals; Escherichia coli/genetics; Genetic Vectors; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Serine Endopeptidases/genetics; Snake Venoms/enzymology; Temperature

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Collection: 01-internacional Database: MEDLINE Main subject: Snake Venoms / Snakes / Thioredoxins / Serine Endopeptidases / Protein Disulfide-Isomerases Limits: Animals Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2004 Document type: Article Affiliation country: China

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