Human osteoblast response to pulsed laser deposited calcium phosphate coatings.
Biomaterials
; 26(15): 2381-9, 2005 May.
Article
in En
| MEDLINE
| ID: mdl-15585241
ABSTRACT
Octacalcium phosphate (OCP) and Mn(2+)-doped carbonate hydroxyapatite (Mn-CHA) thin films were deposited on pure, highly polished and chemically etched Ti substrates with pulsed laser deposition. The coatings exhibit different composition, crystallinity and morphology that might affect their osteoconductivity. Human osteoblasts were cultured on the surfaces of OCP and Mn-CHA thin films, and the cell attachment, proliferation and differentiation were evaluated up to 21 days. The cells showed a normal morphology and a very good rate of proliferation and viability in every experimental time. Alkaline phosphatase activity was always higher than the control and Ti groups. From days 7 to 21 collagen type I production was higher in comparison with control and Ti groups. The level of transforming growth factor beta 1 (TGF-beta1) was lower at 3 and 7 days, but reached the highest values during following experimental times (14 and 21 days). Our data demonstrate that both calcium phosphate coatings favour osteoblasts proliferation, activation of their metabolism and differentiation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Titanium
/
Calcium Phosphates
/
Lasers
Type of study:
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
Biomaterials
Year:
2005
Document type:
Article
Affiliation country:
Italy