Protein open-access liquid chromatography/mass spectrometry.
Rapid Commun Mass Spectrom
; 19(2): 241-9, 2005.
Article
in En
| MEDLINE
| ID: mdl-15609371
Each year increasing numbers of proteins are submitted for routine characterization by liquid chromatography/mass spectrometry (LC/MS). This paper reports a solution that transforms routine LC/MS analysis of proteins into a fully automated process that significantly reduces analyst intervention. The solution developed, protein open-access (OA) LC/MS, consists of web-enabled sample submission and registration, automated data processing, data interpretation, and report generation. Sample submissions and results are recorded in a LIMS that utilizes an Oracle database. The protein sequence is captured during the sample submission process, stored in the database, and utilized to determine the theoretical protein molecular weight. This calculated mass is used to set the parameters for transformation of the mass-to-charge spectra to the mass domain and evaluate the presence or absence of the desired protein. Three protein OA-LC/MS instruments have been deployed in our facility to support protein characterization, purification, and modification efforts.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Mapping
/
Spectrometry, Mass, Electrospray Ionization
/
Myoglobin
Limits:
Humans
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2005
Document type:
Article
Affiliation country:
United States
Country of publication:
United kingdom