High-performance liquid chromatography measurement of hyperforin and its reduced derivatives in rodent plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
; 816(1-2): 21-7, 2005 Feb 25.
Article
in En
| MEDLINE
| ID: mdl-15664329
A reverse-phase high-performance liquid chromatography method was developed for the determination of hyperforin and its reduced derivatives octahydrohyperforin and tetrahydrohyperforin in rodent plasma. The procedure includes solid-phase extraction from plasma using the Baker 3cc C8 cartridge, resolution on the Symmetry Shield RP8 column (150 mm x 4.6 mm, i.d. 3.5 microm) and UV absorbance detection at 300 nm. The assay was linear over a wide range, with an overall coefficient of variation less than 10% for all compounds. The precision and accuracy were within acceptable limits and the limit of quantitation was sufficient for studies preliminarily assessing the disposition of tetrahydrohyperforin and octahydrohyperforin in the mouse and rat.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phloroglucinol
/
Terpenes
/
Bridged Bicyclo Compounds
/
Chromatography, High Pressure Liquid
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2005
Document type:
Article
Affiliation country:
Italy
Country of publication:
Netherlands