Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis.
FEMS Immunol Med Microbiol
; 43(2): 301-10, 2005 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-15681162
ABSTRACT
Bacillus anthracis has four plasmid possible virulence genotypes pXO1+/pXO2+, pXO1+/pXO2-, pXO1-/pXO2+ or pXO1-/pXO2-. Due to the lack of a specific chromosomal marker for B. anthracis, differentiation of the pXO1-/pXO2- form of B. anthracis from closely related Bacillus cereus group species is difficult. In this study, we evaluate the ability of sspE, pXO1 and pXO2 primers to discriminate individual B. anthracis and the B. cereus group genotypes using multiplex real-time PCR and melting curve analysis. Optimal conditions for successful multiplex assays have been established. Purified DNAs from 38 bacterial strains including 11 strains of B. anthracis and 18 B. cereus group strains were analyzed. Nine of the B. cereus group near-neighbor strains were shown by multilocus sequence typing to be phylogenetically proximate to the B. anthracis clade. We have demonstrated that the four plasmid genotypes of B. anthracis and B. cereus group near-neighbors were differentially and simultaneously discriminated by this assay.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacillus anthracis
/
Bacillus cereus
/
Polymerase Chain Reaction
/
Bacteriological Techniques
Type of study:
Diagnostic_studies
Language:
En
Journal:
FEMS Immunol Med Microbiol
Journal subject:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2005
Document type:
Article
Affiliation country:
South Korea