Study of the constitutively active form of the alpha subunit of rice heterotrimeric G proteins.
Plant Cell Physiol
; 46(2): 381-6, 2005 Feb.
Article
in En
| MEDLINE
| ID: mdl-15695461
ABSTRACT
We used site-directed mutagenesis to engineer two constitutively active forms of the alpha subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the alpha subunit was introduced into the rice mutant d1, which is defective for the alpha-subunit gene. All the transformants essentially showed a wild-type phenotype compared with normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
GTP-Binding Protein alpha Subunits
Language:
En
Journal:
Plant Cell Physiol
Journal subject:
BOTANICA
Year:
2005
Document type:
Article