Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme.

Cass, Brian; Pham, Phuong Lan; Kamen, Amine; Durocher, Yves

Cass, Brian; Pham, Phuong Lan; Kamen, Amine; Durocher, Yves.

Affiliation

Cass B; Animal Cell Technology Group, Bioprocess Sector, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Que., Canada H4P 2R2.

Protein Expr Purif ; 40(1): 77-85, 2005 Mar.

Article in En | MEDLINE | ID: mdl-15721774

Subject(s)

Chromatography, Affinity/methods; Recombinant Proteins/isolation & purification; Alkaline Phosphatase/biosynthesis; Alkaline Phosphatase/genetics; Alkaline Phosphatase/isolation & purification; Amino Acid Sequence; Base Sequence; Cells, Cultured; Genetic Vectors; Humans; Luminescent Proteins/biosynthesis; Luminescent Proteins/genetics; Luminescent Proteins/isolation & purification; Molecular Sequence Data; Plasmids/genetics; Recombinant Proteins/biosynthesis; Recombinant Proteins/genetics; Transfection; Vascular Endothelial Growth Factor A/biosynthesis; Vascular Endothelial Growth Factor A/genetics; Vascular Endothelial Growth Factor A/isolation & purification; Red Fluorescent Protein

Search on Google

Add to My VHL

XML

PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Chromatography, Affinity Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2005 Document type: Article Country of publication: United States

Search on Google

Add to My VHL

XML

PubMed Links