Immuno-gene therapy of melanoma by tumor antigen epitope modified IFN-gamma.
Cancer Immunol Immunother
; 54(8): 741-9, 2005 Aug.
Article
in En
| MEDLINE
| ID: mdl-15726359
ABSTRACT
Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-gamma in the same gene by replacing the IFN-gamma signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-gamma on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-gamma decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-gamma transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin Neoplasms
/
Genetic Therapy
/
Interferon-gamma
/
Cancer Vaccines
/
Immunotherapy
/
Melanoma
/
Antineoplastic Agents
Limits:
Animals
Language:
En
Journal:
Cancer Immunol Immunother
Journal subject:
ALERGIA E IMUNOLOGIA
/
NEOPLASIAS
/
TERAPEUTICA
Year:
2005
Document type:
Article
Affiliation country:
United States