Behavior of bacteria in the inductively coupled plasma: atomization and production of atomic ions for mass spectrometry.

ABSTRACT

The combination of perfusion chromatography (PC) and inductively coupled plasma mass spectrometry (ICPMS) is a fast, general way to monitor metal incorporation into bacteria. U+ signals from U incorporated intrinsically in Bacillus subtilis (B. subtilis) are measured with 4 ms time resolution to investigate the behavior of individual cells in the ICP. When intact B. subtilis cells are introduced directly into the ICP, occasional U+ spikes are observed. The positive U+ spikes suggest that bacteria behave more like solid particles than wet droplets in the ICP, compared to previous studies of such transient effects in the ICP. Drying the bacterial aerosol does not eliminate the spikes. Lysing the bacteria by sonication increases the U+ response by 30% compared to that from the untreated sample. PC results from a 10 ppb U standard, partially lysed and fully lysed bacteria samples show that the intracellular U-bound species are released by sonication and are small in size. The atomization-ionization efficiencies for different elements (U, Ca, and Mg) from cells differ somewhat. Reducing the aerosol gas flow rate by 0.1 L min-1 improves the relative U+ response for unlysed bacteria to 85% of that for lysed cells, although the absolute U+ signal is attenuated greatly.