Measurement of 3-hydroxyisovaleric acid in urine of biotin-deficient infants and mice by HPLC.
J Nutr
; 135(3): 615-8, 2005 Mar.
Article
in En
| MEDLINE
| ID: mdl-15735103
ABSTRACT
We developed an assay for measuring urinary 3-hydroxyisovaleric acid (3-HIA) using HPLC after derivatization with 2-nitrophenylhydrazine hydrochloride (2-NPH . HCl). The derivatized 3-HIA was extracted into n-hexane and separated isocratically on a C8 reversed-phase column for fatty acids (YMC-Pack FA). We used this method to measure 3-HIA in urine extracts from mice fed a biotin-deficient diet for >4 wk and in an infant who was fed a special Japanese formula and was suspected of being biotin deficient. Urinary 3-HIA could be assayed within the range of 0.42-8.5 mmol/L with high accuracy by this method, as an indicator of biotin deficiency. Therefore, the HPLC method for 3-HIA described here may be a useful tool clinically as well as in the research laboratory.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Valerates
/
Biotin
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
/
Infant
/
Male
Language:
En
Journal:
J Nutr
Year:
2005
Document type:
Article
Affiliation country:
Japan