Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice.
Can J Vet Res
; 69(1): 53-7, 2005 Jan.
Article
in En
| MEDLINE
| ID: mdl-15745223
ABSTRACT
The aim of this study was to evaluate the effect of 3 Brucella ovis subcellular protein fractions Outer membrane (OMP), inner membrane (IMP), and cytoplasm (CP), on cellular immune response by in vitro production of interleukin (IL)-2, IL-4, and interferon (IFN)-gamma. Each fraction was inoculated 3 times into Balb/c mice, primary cultures of mice spleen cells were done, and these were then stimulated with the fractions. Culture supernatants were collected at 24, 48, 72, 96, and 120 h postinoculation. Cytokine concentration was measured by Duoset-enzyme-linked immunosorbent assay (ELISA). The OMP fraction induced highest cellular immune response of 1000 pg/mL of IL-2 at 24 h, which decreased to < 100 pg/mL by 96 h. The IL-2 response for the IMP fraction was low at 24 h, but exceeded that of the OMP fraction at 72, 96, and 120 h. The CP showed a poor IL response. Regarding the IFN-gamma production, OMP and IMP induced a high response at 120 h. These results open the possibility for the use of B. ovis outer and inner membrane proteins as a subcellular vaccine.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
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Interleukin-4
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Interferon-gamma
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Interleukin-2
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Brucella ovis
Limits:
Animals
Language:
En
Journal:
Can J Vet Res
Journal subject:
MEDICINA VETERINARIA
Year:
2005
Document type:
Article
Affiliation country:
Mexico