'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins.
J Gen Virol
; 86(Pt 4): 1077-1081, 2005 Apr.
Article
in En
| MEDLINE
| ID: mdl-15784901
ABSTRACT
Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Recombination, Genetic
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Rinderpest virus
/
Viral Proteins
/
Genome, Viral
/
Measles virus
Limits:
Animals
/
Humans
Language:
En
Journal:
J Gen Virol
Year:
2005
Document type:
Article
Affiliation country:
United kingdom