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Direct detection of Nocardia spp. in clinical samples by a rapid molecular method.
Couble, Andrée; Rodríguez-Nava, Verónica; de Montclos, Michèle Pérouse; Boiron, Patrick; Laurent, Frédéric.
Affiliation
  • Couble A; UMR CNRS 5557, Center for Microbial Ecology, Opportunistic Pathogens and Environment Research Group, Observatoire Français des Nocardioses (OFN), Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon, France.
J Clin Microbiol ; 43(4): 1921-4, 2005 Apr.
Article in En | MEDLINE | ID: mdl-15815019
ABSTRACT
We developed a 16S PCR-based assay for the rapid detection of Nocardia spp. directly from human clinical samples. The applicability of the assay was confirmed by using 18 samples from patients with nocardiosis as diagnosed by conventional cultures and 20 clinical samples from patients with confirmed tuberculosis used as negative controls.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / RNA, Ribosomal, 16S / Polymerase Chain Reaction / Nocardia / Nocardia Infections Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2005 Document type: Article Affiliation country: France
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / RNA, Ribosomal, 16S / Polymerase Chain Reaction / Nocardia / Nocardia Infections Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2005 Document type: Article Affiliation country: France