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Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium.
Khamis, Atieh; Raoult, Didier; La Scola, Bernard.
Affiliation
  • Khamis A; Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerrannée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
J Clin Microbiol ; 43(4): 1934-6, 2005 Apr.
Article in En | MEDLINE | ID: mdl-15815024
Higher proportions (91%) of 168 corynebacterial isolates were positively identified by partial rpoB gene determination than by that based on 16S rRNA gene sequences. This method is thus a simple, molecular-analysis-based method for identification of corynebacteria, but it should be used in conjunction with other tests for definitive identification.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA-Directed RNA Polymerases / RNA, Ribosomal, 16S / Sequence Analysis, DNA / Corynebacterium / Genes, rRNA Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2005 Document type: Article Affiliation country: France Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA-Directed RNA Polymerases / RNA, Ribosomal, 16S / Sequence Analysis, DNA / Corynebacterium / Genes, rRNA Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2005 Document type: Article Affiliation country: France Country of publication: United States