Protein kinase C theta is not essential for T-cell-mediated clearance of murine gammaherpesvirus 68.
J Virol
; 79(11): 6808-13, 2005 Jun.
Article
in En
| MEDLINE
| ID: mdl-15890920
ABSTRACT
Murine gammaherpesvirus 68 (MHV-68) is a naturally occurring rodent pathogen with significant homology to human pathogens Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. T cells are essential for primary clearance of MHV-68 and survival of mice following intranasal infection. Previous reports have suggested that protein kinase C theta (PKCtheta) is essential for T-cell activation and cytokine production in vitro. To determine the role of this molecule in vivo during the immune response to a viral infection, PKCtheta-/- mice were infected with MHV-68. Despite the essential role of T cells in viral clearance, PKCtheta-/- mice survived infection, cleared lytic virus, and maintained effective long-term control of latency. CD8 T-cell expansion, trafficking to the lung, and cytotoxic activity were similar in PKCtheta+/+ and PKCtheta-/- mice, whereas antiviral antibody and T-helper cell cytokine production were significantly lower in PKCtheta-/- mice than in PKCtheta+/+ mice. These studies demonstrate a differential requirement for PKCtheta in the immune response to MHV-68 and show that PKCtheta is not essential for the T-cell activation events leading to viral clearance.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Kinase C
/
T-Lymphocytes
/
Gammaherpesvirinae
/
Herpesviridae Infections
/
Isoenzymes
Limits:
Animals
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Female
/
Humans
Language:
En
Journal:
J Virol
Year:
2005
Document type:
Article
Affiliation country:
United States