Phosphorylation of the yeast choline kinase by protein kinase C. Identification of Ser25 and Ser30 as major sites of phosphorylation.

Choi, Mal-Gi; Kurnov, Vladlen; Kersting, Michael C; Sreenivas, Avula; Carman, George M

Choi, Mal-Gi; Kurnov, Vladlen; Kersting, Michael C; Sreenivas, Avula; Carman, George M.

Affiliation

Choi MG; Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University, New Brunswick, New Jersey 08901, USA.

J Biol Chem ; 280(28): 26105-12, 2005 Jul 15.

Article in En | MEDLINE | ID: mdl-15919656

Subject(s)

Protein Kinase C/metabolism; Serine/chemistry; Adenosine Triphosphate/chemistry; Alanine/chemistry; Alleles; Amino Acid Motifs; Binding Sites; Biochemical Phenomena; Biochemistry; Choline Kinase/chemistry; Cyclic AMP-Dependent Protein Kinases/chemistry; Cyclic AMP-Dependent Protein Kinases/genetics; DNA/chemistry; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Hydrolysis; Immunoblotting; Immunoglobulin G/chemistry; Immunoprecipitation; Kinetics; Models, Biological; Mutation; Peptide Mapping; Peptides/chemistry; Phosphatidylcholines/chemistry; Phosphorylation; Plasmids/metabolism; Polymerase Chain Reaction; Protein Binding; Protein Kinase C/chemistry; Protein Structure, Tertiary; Saccharomyces cerevisiae; Substrate Specificity; Time Factors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serine / Protein Kinase C Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: J Biol Chem Year: 2005 Document type: Article Affiliation country: United States

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