Localisation of fucoxanthin chlorophyll a/c-binding polypeptides of the centric diatom Cyclotella cryptica by immuno-electron microscopy.
Protoplasma
; 225(3-4): 217-23, 2005 Oct.
Article
in En
| MEDLINE
| ID: mdl-15997336
ABSTRACT
Antisera were raised against the C termini of three fucoxanthin chlorophyll a/c-binding polypeptides, Fcp2, Fcp4, and Fcp6, of the centric diatom Cyclotella cryptica. Immunogold electron microscopy of ultrathin-sectioned cells indicated that Fcp2 and Fcp4 are present in almost the same amounts, whereas approximately 8- to 10-fold less gold label was registered for Fcp6. Immunogold electron microscopy of freeze-fracture replicas of thylakoid membranes showed that the C termini of at least Fcp2 and Fcp4 were located in the thylakoid lumen, thus demonstrating a 3-dimensional structure similar to that already described for the chlorophyll a/b-binding light-harvesting polypeptides of higher plants.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diatoms
/
Light-Harvesting Protein Complexes
Limits:
Animals
Language:
En
Journal:
Protoplasma
Journal subject:
BIOLOGIA
Year:
2005
Document type:
Article
Affiliation country:
Germany