Characterization of a putative calcium-binding site in tobacco mosaic virus.
Proteins
; 12(2): 128-32, 1992 Feb.
Article
in En
| MEDLINE
| ID: mdl-1603802
ABSTRACT
Lead has been used as a substitute for calcium binding to tobacco mosaic virus (TMV). The high atomic number of lead has allowed us to use difference maps from X-ray fiber diffraction data to characterize a calcium-binding site in the virus. The metal ligands are slightly different from those previously believed to bind calcium to TMV, although the binding site is very close to one previously described. Two acetate groups are also bound to the lead atom. There is no significant backbone conformational change in the protein as a result of metal binding; the binding is accomplished by means of relatively small movements in amino acid side chains.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tobacco Mosaic Virus
/
Calcium
/
Lead
Language:
En
Journal:
Proteins
Journal subject:
BIOQUIMICA
Year:
1992
Document type:
Article
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA