Unexpected elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases.
Biosci Biotechnol Biochem
; 69(7): 1418-21, 2005 Jul.
Article
in En
| MEDLINE
| ID: mdl-16041151
ABSTRACT
Mutant strains of Escherichia coli lacking DsbA, DsbB, or DsbD (proteins required for disulfide bond formation in the periplasm) did not produce mitochondrial or chloroplast cytochromes c, as previously observed for bacterial ones. Unexpectedly, however, cytochrome c(555) (AA c(555)) from a hyperthermophile, Aquifex aeolicus, was produced in the E. coli periplasm without Dsb proteins, three times more than with them. These results indicate that the Dsb proteins are not necessarily required for AA c(555) production in E. coli, possibly because of hyperthermophilic origin compared with the others.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxidoreductases
/
Protein Disulfide-Isomerases
/
Cytochrome c Group
/
Escherichia coli
Language:
En
Journal:
Biosci Biotechnol Biochem
Journal subject:
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2005
Document type:
Article