A novel HPLC procedure for detection and quantification of aminoacetone, a precursor of methylglyoxal, in biological samples.
J Chromatogr B Analyt Technol Biomed Life Sci
; 824(1-2): 116-22, 2005 Sep 25.
Article
in En
| MEDLINE
| ID: mdl-16046286
ABSTRACT
Increase in methylglyoxal is thought to be involved in different pathological conditions. Deamination of aminoacetone by semicarbazide-sensitive amine oxidase (SSAO) leads to production of methylglyoxal. We have synthesized aminoacetone and developed a novel HPLC procedure for its quantitative determination. The urinary excretion of aminoacetone is approximately 20-30 microg/mouse/day, and the concentration is about 0.5 microg/g in mouse liver and small intestine. SSAO inhibitor increases aminoacetone levels in both tissues and urines. Results confirm that aminoacetone is an endogenous substrate for SSAO. However, data also indicate that deamination is not the only catabolic pathway for aminoacetone.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acetone
/
Pyruvaldehyde
/
Chromatography, High Pressure Liquid
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2005
Document type:
Article
Affiliation country:
Canada