Expression of bacterial endonucleases in Saccharomyces cerevisiae mitochondria.
Mitochondrion
; 2(1-2): 47-57, 2002 Nov.
Article
in En
| MEDLINE
| ID: mdl-16120308
ABSTRACT
Expression vectors were created in which the 5' end of the Saccharomyces cerevisiae CDC9 gene, which encodes a mitochondrial targeting peptide, was cloned in-frame with the coding regions of the EcoR I, Hind III, and Pst I endonuclease genes. Expression of the EcoR I and Hind III fusion proteins inhibited growth of yeast on glycerol-containing media and resulted in the nearly quantitative restriction digestion of their mitochondrial DNA. In contrast, expression of Pst I, which does not recognize any sites within yeast mitochondrial DNA, had no effect on growth in glycerol-containing media, and did not affect the integrity of the mitochondrial genome.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Mitochondrion
Year:
2002
Document type:
Article
Affiliation country:
United States