Near infrared two-photon excitation cross-sections of voltage-sensitive dyes.
J Neurosci Methods
; 148(1): 94-102, 2005 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-16129493
ABSTRACT
Microscopy based on voltage-sensitive dyes has proven effective for revealing spatio-temporal patterns of neuronal activity in vivo and in vitro. Two-photon microscopy using voltage-sensitive dyes offers the possibility of wide-field visualization of membrane potential on sub-cellular length scales, hundreds of microns below the tissue surface. Very little information is available, however, about the utility of voltage-sensitive dyes for two-photon imaging purposes. Here we report on measurements of two-photon fluorescence excitation cross-sections for nine voltage-sensitive dyes in a solvent, octanol, intended to simulate the membrane environment. Ultrashort light pulses from a Tisapphire laser were used for excitation from 790 to 960 nm, and fluorescein dye was used as a calibration standard. Overall, dyes RH795, RH421, RH414, di-8-ANEPPS, and di-8-ANEPPDHQ had the largest two-photon excitation cross-sections ( approximately 15 x 10(-50)cm4 s photon(-1)) in this wavelength region and are therefore potentially useful for two-photon microscopy. Interestingly, di-8-ANEPPDHQ, a chimera constructed from the potentiometric dyes RH795 and di-8-ANEPPS, exhibited larger cross-sections than either of its constituents.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Photons
/
Spectroscopy, Near-Infrared
/
Fluorescent Dyes
/
Neurons
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Neurosci Methods
Year:
2005
Document type:
Article
Affiliation country:
United States