A high-throughput method for screening of rapamycin-producing strains of Streptomyces hygroscopicus by cultivation in 96-well microtiter plates.
Biotechnol Lett
; 27(15): 1135-40, 2005 Aug.
Article
in En
| MEDLINE
| ID: mdl-16132865
ABSTRACT
A novel high-throughput cultivation method was developed to rapidly screen large numbers of rapamycin-producing mutants of Streptomyces hygroscopicus by duplicate culturing of isolates on the surfaces of agar-solidified 96 wells in microtiter plates. One copy of the cultures was used for the rapamycin bioassay and the other identical copy, representing potentially high yielding strains, was preserved for further study. By integrating 96-well solid cultivation and the bioassay, we screened more than 7000 isolates and found 10 high-yielding strains. From these, one mutant produced 420 mug rapamycin/ml, which was double the yield of parent strain used in the submerged fermentation process.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptomyces
/
Biotechnology
/
Sirolimus
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
Biotechnol Lett
Year:
2005
Document type:
Article
Affiliation country:
China