[Experimental techniques for ancient DNA research].

ABSTRACT

The development of modern molecular biological techniques makes it possible to study minimum DNA from ancient materials. During past decade,a lot of significant achievements on ancient DNA research have been made in many fields especially in molecular evolutionary biology. The nature of degradation and contamination of ancient DNA from ancient biological materials pose a dominating problem in ancient DNA research. Therefore,the experiments should be modified based on the modern molecular techniques and more factors should be considered when the results are analyzed. In this paper,authors review the general experimental protocols on sampling,extraction and amplification as well as authenticity of ancient DNA.