Dysfunction of erythropoietin-producing interstitial cells in the kidneys of ICR-derived glomerulonephritis (ICGN) mice.
J Vet Med Sci
; 67(9): 891-9, 2005 Sep.
Article
in En
| MEDLINE
| ID: mdl-16210801
Anemia is a major secondary symptom in chronic renal disorder (CRD), but the precise cause of insufficient production of erythropoietin (EPO) remains unclear owing to the controversial localization of EPO-producing cells in the kidneys. The ICR-derived glomerulonephritis (ICGN) mouse, a new hereditary nephrotic mouse, is an appropriate model of anemia associated with CRD. By using an amplified in situ hybridization technique, we detected and counted the renal EPO-producing cells under both normoxic and hypoxic conditions. The expression levels of renal EPO mRNA were quantified and oxygen gradients were also assessed immunohistochemically. Amplified in situ hybridization clarified that EPO-producing cells were peritubular interstitial cells in the middle region of renal cortex in both ICR and ICGN mice. Hypoxia (7% O2) induced low oxygen tension in proximal tubular epithelial cells of renal cortex, and increased the expression of EPO mRNA and the number of EPO-producing cells in both ICR and ICGN mice. However, hypoxia did not increase the serum EPO levels in ICGN mice. The ICGN mouse is a good model for anemia associated with CRD, and the suppression of EPO protein production in the renal EPO-producing cells is considered to be a potential cause of anemia associated with CRD.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Erythropoietin
/
Anemia
/
Kidney
/
Kidney Failure, Chronic
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
J Vet Med Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2005
Document type:
Article
Affiliation country:
Japan
Country of publication:
Japan