Your browser doesn't support javascript.
loading
Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming.
Smith, Dorie; Zhong, Jin; Matsuura, Manabu; Lambowitz, Alan M; Belfort, Marlene.
Affiliation
  • Smith D; Molecular Genetics Program, Wadsworth Center, New York State Department of Health and School of Public Health, State University of New York at Albany, Albany, New York 12201, USA.
Genes Dev ; 19(20): 2477-87, 2005 Oct 15.
Article in En | MEDLINE | ID: mdl-16230535
Retrohoming of group II introns occurs by a mechanism in which the intron RNA reverse splices directly into one strand of a DNA target site and is then reverse transcribed by the associated intron-encoded protein. Host repair enzymes are predicted to complete this process. Here, we screened a battery of Escherichia coli mutants defective in host functions that are potentially involved in retrohoming of the Lactococcus lactis Ll.LtrB intron. We found strong (greater than threefold) effects for several enzymes, including nucleases directed against RNA and DNA, replicative and repair polymerases, and DNA ligase. A model including the presumptive roles of these enzymes in resection of DNA, degradation of the intron RNA template, traversion of RNA-DNA junctions, and second-strand DNA synthesis is described. The completion of retrohoming is viewed as a DNA repair process, with features that may be shared by other non-LTR retroelements.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / DNA, Bacterial / Introns / Retroelements / Escherichia coli Type of study: Prognostic_studies Language: En Journal: Genes Dev Journal subject: BIOLOGIA MOLECULAR Year: 2005 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / DNA, Bacterial / Introns / Retroelements / Escherichia coli Type of study: Prognostic_studies Language: En Journal: Genes Dev Journal subject: BIOLOGIA MOLECULAR Year: 2005 Document type: Article Affiliation country: United States Country of publication: United States