Characterisation and biological activity of monoclonal antibodies specific for Pasteurella haemolytica A1 capsule and lipopolysaccharide.
Vet Microbiol
; 31(2-3): 161-8, 1992 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-1626366
ABSTRACT
Monoclonal antibodies (mAb) against both Pasteurella haemolytica A1 capsule and lipopolysaccharide (LPS) were produced. Anti-capsule mAb reacted with the homologous A1 serotype only, whereas mAb against LPS reacted with P. haemolytica serotypes A2, A5, A8, A12, A14 and A16 but not with 33 bacterial species or rough LPS mutant strains tested. Both capsule and LPS antigens were visualised on the surface of bacteria by immunogold electron microscopy. Neither of the mAbs demonstrated antibody-dependent complement-mediated killing in vitro but both facilitated phagocytosis in vitro.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lipopolysaccharides
/
Mannheimia haemolytica
/
Bacterial Capsules
/
Antibodies, Monoclonal
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
1992
Document type:
Article
Affiliation country:
United kingdom
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS