The beta-tubulin genes of two Strongyloides species.
Exp Parasitol
; 112(3): 144-51, 2006 Mar.
Article
in En
| MEDLINE
| ID: mdl-16309677
ABSTRACT
The World Health Organization is sponsoring major treatment programs with the aim of controlling helminth infection throughout the tropical world. Prominent among the anthelmintics recommended for use in these programs are drugs in the benzimidazole (BZ) class. Resistance to these drugs has been associated with polymorphisms in the beta-tubulin gene. We have cloned and sequenced the beta-tubulin genes of Strongyloides stercoralis and Strongyloides ratti and have proceeded to develop a protocol for genotyping single worms for polymorphisms in beta-tubulin. Our findings indicate that S. ratti has a single beta-tubulin gene, making DNA sequence analysis of a single larva PCR product a feasible means of studying BZ resistance in these species. Our genotyping test allows the identification of polymorphisms at codons 167, 198, and 200 in the Strongyloides beta-tubulin gene, thus enabling survey for BZ resistant genotypes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tubulin
/
Strongyloides stercoralis
/
Strongyloides ratti
/
DNA, Complementary
/
DNA, Helminth
Type of study:
Guideline
/
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Exp Parasitol
Year:
2006
Document type:
Article
Affiliation country:
Australia