Development of a real time RT-PCR to detect and type ovine pestiviruses.
J Virol Methods
; 132(1-2): 187-94, 2006 Mar.
Article
in En
| MEDLINE
| ID: mdl-16309752
ABSTRACT
A real time one-step RT-PCR was designed to detect and type border disease virus (BDV), bovine viral diarrhea virus (BVDV) type 1 and BVDV type 2 in ovine samples. The real time RT-PCR was shown to behave in a linear manner and had limits of detection of 100-1000 copies of viral RNA as judged by in vitro transcribed RNA. The real time RT-PCR was validated on 50 clinical samples from UK flocks and was more sensitive than a virus isolation and a classical nested RT-PCR (nRT-PCR). The results of real time RT-PCR virus typing agreed completely with sequencing. The majority of ovine isolates were BDV; a small proportion were BVDV type 1. BVDV type 2 was not detected in any sample. This test appears reliable and can be used for the typing of ovine pestiviruses in the UK.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Border Disease
/
Pestivirus Infections
/
Border disease virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diarrhea Virus 1, Bovine Viral
/
Diarrhea Virus 2, Bovine Viral
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
J Virol Methods
Year:
2006
Document type:
Article
Affiliation country:
United kingdom