[Detection of the Bartonella DNA by the method of nested PCR in patients after tick bites in Novosibirsk region].

Morozova, O V; Chernousova, N Ia; Morozov, I V

Morozova, O V; Chernousova, N Ia; Morozov, I V.

Mol Gen Mikrobiol Virusol ; (4): 14-7, 2005.

Article in Ru | MEDLINE | ID: mdl-16334219

ABSTRACT

ABSTRACT

Arthropod-borne bacterial pathogen Bartonella DNA was detected in human blood cells after tick bites in summer 2003 and 2004 in Novosibirsk region by nested PCR with primers specific to groEL gene of HSP60 protein. Comparative assay of 190 p.b. of long PCR fragment revealed that the nucleotide sequences might belong to Bartonella henselae and Bartonella quintana.

Subject(s)

Bartonella Infections/microbiology; Bartonella/genetics; DNA, Bacterial/analysis; Polymerase Chain Reaction/methods; Animals; Bartonella/isolation & purification; Bartonella Infections/etiology; Bartonella henselae/genetics; Bartonella henselae/pathogenicity; Bartonella quintana/genetics; Bartonella quintana/pathogenicity; Bites and Stings; Blood/microbiology; Humans; Siberia; Ticks

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Collection: 01-internacional Database: MEDLINE Main subject: Bartonella / Bartonella Infections / DNA, Bacterial / Polymerase Chain Reaction Type of study: Diagnostic_studies / Etiology_studies Limits: Animals / Humans Country/Region as subject: Asia Language: Ru Journal: Mol Gen Mikrobiol Virusol Journal subject: BIOLOGIA MOLECULAR / MICROBIOLOGIA / VIROLOGIA Year: 2005 Document type: Article

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