Neospora caninum: antibodies directed against tachyzoite surface protein NcSRS2 inhibit parasite attachment and invasion of placental trophoblasts in vitro.
Exp Parasitol
; 112(3): 172-8, 2006 Mar.
Article
in En
| MEDLINE
| ID: mdl-16375893
ABSTRACT
Polyclonal and monoclonal antibodies to native Neospora caninum tachyzoite surface protein NcSRS2 were generated and tested in vitro for their ability to neutralize tachyzoite attachment to and invasion of host cells. Host cells included Vero cells and a newly cloned, immortalized ovine trophoblast cell line obtained from primary cultures of ovine placenta. The ovine trophoblasts had morphology consistent with fetal trophoblasts and expressed mRNA for interferon-tau, a marker for trophoblasts. Native NcSRS2 was used to immunize mice to obtain monospecific anti-NcSRS2 polyclonal serum and anti-NcSRS2 monoclonal antibodies. Compared to irrelevant antibodies, monospecific anti-NcSRS2 serum and two anti-NcSRS2 monoclonal antibodies, 100.2.4.4 and 119.4.9.10, significantly blocked invasion of tachyzoites into both trophoblasts and Vero cells. Parasite attachment, assessed by IFA, was significantly reduced by anti-NcSRS2 mAb 100.2.4.4 and monospecific serum. The findings provide rationale to investigate a role for antibodies to NcSRS2 in prevention of N. caninum transplacental transmission in vivo.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Placenta
/
Trophoblasts
/
Protozoan Proteins
/
Neospora
/
Antibodies, Monoclonal
/
Antigens, Protozoan
/
Antigens, Surface
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Exp Parasitol
Year:
2006
Document type:
Article
Affiliation country:
United States