A versatile tool for conditional gene expression and knockdown.

Szulc, Jolanta; Wiznerowicz, Maciej; Sauvain, Marc-Olivier; Trono, Didier; Aebischer, Patrick

Szulc, Jolanta; Wiznerowicz, Maciej; Sauvain, Marc-Olivier; Trono, Didier; Aebischer, Patrick.

Affiliation

Szulc J; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Nat Methods ; 3(2): 109-16, 2006 Feb.

Article in En | MEDLINE | ID: mdl-16432520

Subject(s)

Gene Expression Regulation/genetics; Gene Targeting/methods; RNA Interference; Animals; Bacterial Proteins/genetics; Brain/metabolism; Carrier Proteins/genetics; Cell Differentiation/genetics; Cell Line; Cell Line, Tumor; DNA Polymerase II/genetics; DNA Polymerase III/genetics; Doxycycline/pharmacology; Female; GATA1 Transcription Factor/genetics; Gene Expression Regulation/drug effects; Gene Silencing; Genetic Engineering/methods; Genetic Vectors/genetics; Glial Cell Line-Derived Neurotrophic Factor/genetics; Glial Cell Line-Derived Neurotrophic Factor/metabolism; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Humans; Lentivirus/genetics; Male; Mice; Mice, Inbred Strains; Mice, Nude; Mice, Transgenic; Promoter Regions, Genetic/genetics; RNA, Small Interfering/genetics; Rats; Rats, Sprague-Dawley; Rats, Wistar; Repressor Proteins/genetics; Stem Cells/cytology; Stem Cells/drug effects; Stem Cells/metabolism; Transfection; Transgenes/genetics; Tumor Suppressor Protein p53/genetics; Xenograft Model Antitumor Assays

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Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Regulation / Gene Targeting / RNA Interference Type of study: Prognostic_studies Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2006 Document type: Article Affiliation country: Switzerland Country of publication: United States

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