The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processes.
J Gen Virol
; 87(Pt 3): 613-622, 2006 Mar.
Article
in En
| MEDLINE
| ID: mdl-16476982
ABSTRACT
The complete genome of West Nile (Sarafend) virus [WN(S)V] was sequenced. Phylogenetic trees utilizing the complete genomic sequence, capsid gene, envelope gene and NS5 gene/3' untranslated region of WN(S)V classified WN(S)V as a lineage II virus. A full-length infectious clone of WN(S)V with a point mutation in the glycosylation site of the envelope protein (pWNS-S154A) was constructed. Both growth kinetics and the mode of maturation were affected by this mutation. The titre of the pWNS-S154A virus was lower than the wild-type virus. This defect was corrected by the expression of wild-type envelope protein in trans. The pWNS-S154A virus matured intracellularly instead of at the plasma membrane as shown for the parental WN(S)V.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
West Nile virus
/
Viral Envelope Proteins
/
Genome, Viral
Limits:
Animals
Language:
En
Journal:
J Gen Virol
Year:
2006
Document type:
Article