Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation.
Int Endod J
; 39(3): 238-44, 2006 Mar.
Article
in En
| MEDLINE
| ID: mdl-16507078
ABSTRACT
AIM:
To examine whether low-power laser irradiation (LPLI) promotes cellular proliferation of human dental pulp-derived fibroblast-like cells (dental pulp cells).METHODOLOGY:
Dental pulp cells were obtained by primary culture of human dental pulp tissues from extracted third molar teeth. The phosphorylation of the mitogen-activated protein kinase (MAPK) family after LPLI of these cells was investigated by Western blotting. By using a specific MAPK/ERK kinase (MEK) inhibitor (PD098059), the specific effect of LPLI on the MAPK pathway was also investigated by Western blotting as described above. The incorporation of [3H]thymidine into the cells after LPLI was determined, and statistical analysis was performed by Wilcoxon signed-ranks test.RESULTS:
Extracellular signal-regulated protein kinase (ERK) 1/2 was phosphorylated between 5 and 30 min after LPLI. Moreover, PD098059 inhibited LPLI-mediated ERK1/2 activation. LPLI did not affect p38 MAPK or c-Jun N-terminal kinase (JNK) phosphorylation. But LPLI did not stimulate [3H]thymidine incorporation into these cells.CONCLUSIONS:
These results indicated that LPLI activated MAPK/ERK, a signal for proliferation, differentiation and survival, but did not activate the stress signals p38 MAPK and JNK in human dental pulp cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mitogen-Activated Protein Kinases
/
Dental Pulp
/
Extracellular Signal-Regulated MAP Kinases
/
Laser Therapy
Limits:
Humans
Language:
En
Journal:
Int Endod J
Year:
2006
Document type:
Article
Affiliation country:
Japan